小鼠肺上皮祖细胞系C22

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ECACC #07021401,C22 (Clara),小鼠肺上皮祖细胞系C22 (Clara),1 VIAL,准现货供应原装冻存管

细胞链接:ECACC General Cell Collection: 07021401 C22 (Clara) (culturecollections.org.uk)

产品详细信息如下:

ECACC General Cell Collection: C22 (Clara)


Supplied by:European Collection of Authenticated Cell Cultures (ECACC)
Culture Type:Cell line
Collection:ECACC General Collection

Catalogue No.:07021401
Cell Line Name:C22 (Clara)

Citation Guidance:If use of this culture results in a scientific publication, it should be cited in the publication as: C22 (Clara) (ECACC 07021401)

Keywords:Mouse lung epithelial progenitor cell
Cell Line Description:A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.
Species:Mouse
Tissue of Origin:Lung

CellType:Epithelial

Growth Mode:Adherent
Karyotype:Not

GMO Status:Genetically Modified Organism Class 1 (GMO1)
Biosafety Information:Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet

Subculture Routine:Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO2; 33°C. Doubling time 48h
Culture Medium:DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 supplements:

0.25 μg/ml         Endothelin-1
0.01 μg/ml         Interferon gamma
10 μg/ml           Insulin
5 μg/ml             Transferrin
7.5 μg/ml          Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml       Epidermal Growth Factor
0.36 μg/ml         Hydrocortisone  
0.02 μg/ml         T3



For guidance on the supplements and preparation of the culture medium please refer to the product document: T7 and C22(Clara) Culture Medium.pdf

Depositor:Dr Daphne deMello, Department of Pathology, Phoenix Childrens Hospital, 1919 East Thomas Road, Phoenix, Arizona 85016, USA
Originator:Yes
Country:USA
References:Demello et al., (2002) Generation and characterization of a conditionally immortalized lung Clara cell line from the H-2K In Vitro Cell. Dev. Biol.-Animal 38: 154-164
Additional Bibliography:Not Available
Patents:None Specified By Depositor
Release Conditions:No






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