C57BL/6J小鼠肝细胞癌细胞系Hep-55.1C

Description细胞描述

The Hep-55.1C   cell line is derived from C57BL/6J mice and is a critical tool in the study   of hepatocellular carcinoma (HCC), one of the most common types of liver   cancer. This cell line is utilized primarily to understand the intricacies of   tumor biology, including the mechanisms of tumor initiation, progression, and   metastasis. The Hep-55.1C cells provide a consistent and reproducible model   for evaluating the efficacy of anti-cancer drugs and for exploring the   molecular pathways involved in the disease.

Organism物种

Mouse小鼠

Tissue组织

Liver肝脏

Disease疾病

Hepatocellular carcinoma肝细胞癌

Synonyms同名词

HEP-55.1C, 55.1C

Age年龄段

Adult成年

Gender性别

Female雌性

Morphology形态

Epithelial-like 类上皮细胞

Growth properties生长特性

Adherent贴壁细胞

Citation

Hep-55.1C (Cytion catalog number 400201)

Biosafety level

1

Protein expression

Keratin 8, Keratin 18, Vimentin.

Tumorigenic

Yes, in C57BL/6J mice

Mutational profile

p53 wt

Culture Medium

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5   g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Medium supplements

Supplement the medium with 10% FBS

Passaging solution

Accutase

Subculturing

Remove the old medium from the adherent cells and wash   them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of   PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with   Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the   cells incubate at room temperature for 8-10 minutes to detach them. After   incubation, gently mix the cells with 10 ml of medium to resuspend them, then   centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells   in fresh medium, and transfer them into new flasks that already contain fresh   medium.

Split ratio

A ratio of 1:4 to 1:8 is recommended

Fluid renewal

Every 3 to 5 days

Freezing recovery

Start culture from cryovial at a cell density of 3 to 4   x 10^4 cells/cm^2. The cells will recover within 24 to 48 hours.

Freeze medium

CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion   catalog number 806100)

Handling of cryopreserved cultures

1. Confirm that the vial remains deeply frozen       upon delivery, as cells are shipped on dry ice to maintain optimal       temperatures during transit.

2. Upon receipt, either store the cryovial       immediately at temperatures below -150°C to ensure the preservation of       cellular integrity, or proceed to step 3 if immediate culturing is       required.

3. For immediate culturing, swiftly thaw the vial       by immersing it in a 37°C water bath with clean water and an       antimicrobial agent, agitating gently for 40-60 seconds until a small       ice clump remains.

4. Perform all subsequent steps under sterile       conditions in a flow hood, disinfecting the cryovial with 70% ethanol       before opening.

5. Carefully open the disinfected vial and       transfer the cell suspension into a 15 ml centrifuge tube containing 8       ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes       to separate the cells and carefully discard the supernatant containing       residual freezing medium. Optionally, skip centrifugation but remove any       remaining freezing medium after 24 hours.

7. Gently resuspend the cell pellet in 10 ml of       fresh culture medium. For adherent cells, divide the suspension between       two T25 culture flasks; for suspension cultures, transfer all the medium       into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for       continued growth and maintenance of the cell line, ensuring reliable       experimental outcomes.

Sterility

Mycoplasma   contamination is excluded using both PCR-based assays and luminescence-based   mycoplasma detection methods.

To ensure there   is no bacterial, fungal, or yeast contamination, cell cultures are subjected   to daily visual inspections.

STR profile

M_18-3: 16

M_4-2: 20.3

M_6-7: 17

M_3-2: 14

M_19-2: 13

M_7-1: 26.2,27.2

M_1-1: 16

M_8-1: 16

M_2-1: 15

M_15-3: 22.3

M_6-4: 18

M_11-2: 16

M_1-2: 20

M_17-2: 15

M_12-1: 17

M_5-5: 16,17

M_X-1: 28

M_13-1: 17

Human D4/D8: -