ECACC的97041101,Nb2-11 Cell Line from rat,大鼠Nb2-11细胞系,1 VIAL,准现货供应原装冻存管、复苏细胞。



ECACC General Cell Collection: Nb2-11

Supplied by:European Collection of Authenticated Cell Cultures (ECACC)
Culture Type:Cell line
Collection:ECACC General Collection

Catalogue No.:97041101
Cell Line Name:Nb2-11

Citation Guidance:If use of this culture results in a scientific publication, it should be cited in the publication as: Nb2-11 (ECACC 97041101)

Keywords:Rat lymphoma
Cell Line Description:Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.
Tissue of Origin:thymus/lymph node


Growth Mode:Suspension

Biosafety Information:Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet

Subculture Routine:At resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4x1,000 and 12x1,000 cells/ml for culture periods of 96 and 72 hours respectively. Incubate flasks at 37°C; 5 % CO2. Check daily. Do not exceed 9x100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.
Culture Medium:Fischer's medium (Life Technologies product number : 21475025) + 10%Foetal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Na Bicarbonate + 0.05mM 2-Mercaptoethanol (2ME) + 2mM Glutamine.
Depositor:Dr Peter Gout and Dr Henry Friesen, Department of Cancer Endocrinology, British Columbia Cancer Agency, Vancouver
References:Gout PW, Beer CT, Noble RL 1980 Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas Cancer Res. 40(7):2433-6 PMID: 6992985.
Additional Bibliography:Horsman DE, Masui S, Gout PW 1991 Karyotypic changes associated with loss of prolactin dependency of rat Nb2 node lymphoma cell cultures. Cancer Res.; 51(1):282-7.PMID: 1988090. Horseman ND 1995 Prolactin, proliferation, and protooncogenes Endocrinology. 136(12):5249-51 PMID: 7588267. Gout PW, Horsman DE, Fox K, De Jong G, Ma S, Bruchovsky N. 1994 The rat Nb2 lymphoma: a novel model for tumor progression. Anticancer Res. 14:2485-92. PMID: 7872671
Patents:None specified by Depositor
Release Conditions:No



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